TABLE 1.
Treatment | % of gated population that showed: |
|
---|---|---|
Permeabilizationb | Depolarizationc | |
None | 1.88 | 3.8 |
CCCP (control) | 1.95 | 100 |
Bac8c (5 min)d | 4.56 | 33.5 |
Bac8c (10 min)d | 14.5 | 33.8 |
Bac8c (30 min)d | 35.6 | 52.3 |
Bac8c (60 min)d | 65 | 74 |
Results summarized here correspond to data illustrated in Fig. 2C and D.
The cutoff for permeabilization was 101 labeled cells.
The cutoff for depolarization was a ratio of 400. The ratio corresponds to the ratiometric parameter (red [depolarized cells]/green [polarized cells] used, which was calculated as follows: red value − green value + 384.
Time after Bac8c addition to cultures.