Growth curves of different species of nonfermentative yeast in different media. Fungal growth was recorded during 72 h as described in Materials and Methods. The graph represents the average optical densities of the stains used for each species (n) under the following growth conditions: the CLSI protocol (RPMI medium with 0.2% glucose at 35°C with 0.5 × 103 to 2.5 × 103 CFU/ml and no shaking), the EUCAST protocol (RPMI medium plus 2% glucose at 35°C with 1 × 105 to 5 × 105 CFU/ml and no shaking), the EUCAST protocol at 30°C, the EUCAST protocol at 30°C with shaking (SH), YNB under static (ST) conditions (YNB medium plus 0.5% glucose with 1 × 105 to 5 × 105 CFU/ml at 30°C under static conditions), and YNB under shaking conditions (YNB medium plus 0.5% glucose with 1 × 105 to 5 × 105 CFU/ml at 30°C with shaking during the incubation).