TABLE 2.
Primers used in this study
| Primer | Sequencea | Reference or source |
|---|---|---|
| AHqRTFor | 5′-GGACCGATGCTGGTCTATGT-3′ | This study |
| AHqRTRev | 5′-CAGATAACAGGCCATGACGA-3′ | This study |
| rubA3qRTFor | 5′-CTACCGTGTCCGGTCTGTG-3′ | This study |
| rubA3qRTRev | 5′-CCAGTCGTCGGGAATGTC-3′ | This study |
| rubA4qRTFor | 5′-CTGCGAGGTCTGCGGATT-3′ | This study |
| rubA4qRTRev | 5′-GGCCACCTCGACCATCTC-3′ | This study |
| rubBqRTFor | 5′-GGGTGTTGATCCAGTTCAGG-3′ | This study |
| rubBqRTRev | 5′-TATCTGGCACATCACCAACG-3′ | This study |
| alkUqRTFor | 5′-GCGTTCACCGAGTACTTCAC-3′ | This study |
| alkUqRTRev | 5′-ATCGACAACCACGTCGACTC-3′ | This study |
| alkNIFor | 5′-AACATATGCTCGTGAGAGGAGCGTGC-3′ | This study |
| alkBHIRev | 5′-AAGGATCCCCGGACAACGGTAGGCGC-3′ | This study |
| CF | 5′-ATGTTYATHGCNATGGAYCCNC-′ | 11 |
| CR | 5′-NARNCKRTTNCCCATRCANCKRTG-′ | 11 |
| apra750FR | 5′-ATTCCGGGGATCCGTCGACC-′ | This study |
| apra750RV | 5′-TGTAGGCTGGAGCTGCTTC-′ | This study |
| ladAFR | 5′-GGCGTSTACGMCRWCTACGGYRGG-′ | This study |
| ladARV | 5′-GAYCTACCAGGYCGGGTCGTCG-′ | This study |
| alkCG341FR | 5′-CCGAGGACCCGGCGAGCTC-′ | This study |
| alkCG341RV | 5′-CTCCGGGGTGCACCGCTC-′ | This study |
a
Underlining indicates NdeI (for alkNIFor) and BamHI (for alkBHIRev) restriction sites.