TABLE 2.
Assay performances and cycling conditions of primers used for quantitative real-time PCR
| Primer | Annealing temp (°C) | Primer concn (nM) | Approximate length of PCR product (bp) | Standard templates (GenBank accession numbers) | qPCR performance |
||
|---|---|---|---|---|---|---|---|
| Mean efficiency (%) ± SD | Linearity R2 | Dynamic range (no. of target genes/PCR) | |||||
| DsrA243Fb | 64 | 250 | 330 | AY167467, AY167479, AY167468 | 83.2 ± 3.0 | 0.98 | 102-106 |
| DsrA561Rb | |||||||
| DsrA243Fa | 64 | 250 | 330 | AY167473, AY167466, AY167480 | 89.8 ± 0.4 | 0.99 | 102-105 |
| DsrA561Ra | |||||||
| DsrA216F | 64 | 250 | 360 | AY167478, AY167465 | 73.6 ± 1.4 | 0.98 | 102-106 |
| DsrA561Re | |||||||
| SYBAC836F | 64 | 250 | 160 | X70905, X70906 | 77.5 ± 0.1 | 0.99 | 102-106 |
| SYBAC986R | |||||||
| 1389F | 52 | 750 | 120 | X70905, X70906 | 90.0 ± 2.8 | 1.00 | 102-106 |
| 1492R | 1,000 | ||||||