TABLE 1.

Primer used in this work

Primer no.	Primer name	Sequence	Usagea
1	BspHI_recA_for	CGGCGGTCATGAGTATCGACGAAAACAAACAG	Construction of pKD46 recA
2	HindIII_recA_rev	GCCAAGCTTTTAAAAATCTTCGTTAGTTTC	Construction of pKD46 recA
3	pKD46_exo_int_for	GAGGCACTGGCTGAAATTGG	Sequencing of pKD46 recA
4	recA_int_rev	GTTTACGTGCGTAGATTGGG	Sequencing of pKD46 recA
5	mtrB_NcoI_for	CATGCCATGGATGAAATTTAAACTCAATTT	Construction of pBAD mtrBStrep
6	mtrB_strep_HindIII_rev	GGGAAGCTTTTATTTTTCGAACTGCGGGTGGCTCCAGGCGCCGAGTTTGTAACTCATGCT	Construction of pBAD mtrBStrep
7	ΔmtrA_up_for	GATCCCCGGGTACCGAGCTCGAATTCGTAACATTCCCAGCGGTCGGTC	mtrA knockout mutant
8	ΔmtrA_up_rev	AATAGGCTTCCCAATTTGTCCC	mtrA knockout mutant
9	ΔmtrA_down_for	CGAATTCTGGGACAAATTGGGAAGCCTATTCAGCGCTAAGGAGACGAG	mtrA knockout mutant
10	ΔmtrA_down_rev	AGCTTGCATGCCTGCAGGTCGACTCTAGAGGTTCGAGGGCATTGAGGC	mtrA knockout mutant
11	mtrBgen_up_for	ATGATTACGAATTCGAGCTCGGTACCCGGGGGGGTGGCGGATGAACTGTC	Strep epitope knock-in
12	mtrBgen_up_rev	GGCGCCTGGAGCCACCCGCAGTTCGAAAAATAATCCATTTGCCTCATATGCTC	Strep epitope knock-in
13	mtrBgen_down_for	TTATTTTTCGAACTGCGGGTGGCTCCAGGCGCCGAGTTTGTAACTCATGCT	Strep epitope knock-in
14	mtrBgen_down_rev	CGGCCAGTGCCAAGCTTGCATGCCTGCAGGCAAAAGACACCAGTTATGATG	Strep epitope knock-in
15	ΔdegP_up_for	ATGATTACGAATTCGAGCTCGGTACCCGGGCCATCTCGAGTAAGATCTTTTTG	degP knockout mutant
16	ΔdegP_up_rev	CTATTCATAACTCCAAATAAGGG	degP knockout mutant
17	ΔdegP_down_for	CTTATTTGGAGTTATGAATAGTCAATTGGCGAATCTGATC	degP knockout mutant
18	ΔdegP_down_rev	CGGCCAGTGCCAAGCTTGCATGCCTGCAGGCACTTCAGAGGTGAACTTGC	degP knockout mutant
19	RT_mtrB_for	CGGCTTAAAACAAGCCTCTG	qRT-PCR
20	RT_mtrB_rev	CCAAAGGTGGGGTTAAAAGC	qRT-PCR
21	dnaK_for	ATGGGTAAAATTATTGGTATC	qRT-PCR
22	dnaK_rev	TTATTTCTTGTCGTCTTTCAC	qRT-PCR
23	RT_dnaK_for	CGTGACGTGAACATCATGC	qRT-PCR
24	RT_dnaK_rev	CAGAAACCTGTGGTGGAGC	qRT-PCR
25	RT_gyrB_for	GCTTGATTGAAGTCGGTGGT	qRT-PCR
26	RT_gyrB_rev	CGTTTCGCTTCAGAAATGGT	qRT-PCR
27	RT_recA_for	AGCTATAGCCGCTGAAATCG	qRT-PCR
28	RT_recA_rev	CCTCGACATTGTCATCATCG	qRT-PCR
29	HindIII_mtrA_rev	GGGAAGCTTTTAGCGCTGTAATAGCTTGC	Construction of pRSF mtrA mtrBStrep
30	BspHI_mtrA_for	GAAATATCATGAAGAACTGCCTAAAAATG	Construction of pRSF mtrA mtrBStrep
31	mtrB_NdeI_for	GGAATTCCATATGAAATTTAAACTCAATTTGATC	Construction of pRSF mtrBStrep
32	KpnI_mtrBstrep_rev	CGGGGTACCTTATTTTTCGAACTGCGGGTGGCTCCAGGCGCCGAGTTTGTAACTCATGCT	Construction of pRSF mtrBStrep
33	pRSF_MCS1_for	GGATCTCGACGCTCTCCCT	Sequencing of pRSF mtrA mtrBStrep
34	pRSF_MCS2_for	TTGTACACGGCCGCATAATC	Sequencing of pRSF mtrBStrep
35	BL21_ΔdegP_ rev	CAGATTGTAAGGAGAACCCCTTCCCGTTTTCAGGAAGGGGTTGAGGGAGACTAAGCACTTGTCTCCTGTTT	Construction of conditional degP mutant
36	E.coli_degPtet_for	TTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGTTTAAGACCCACTTTCACATTTAA	Construction of conditional degP mutant

^aqRT-PCR, quantitative reverse transcription-PCR.