TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| Strains | ||
| M. goodii 12523 | Wild type | 20 |
| M. smegmatis mc2155 | Wild type, ATCC 700084 | ATCC |
| M. smegmatis mc2155 ΔmimA | M. smegmatis strain mc2155 with mimA deletion | This study |
| Gordonia sp. strain TY-5 | Wild type | 7 |
| Gordonia sp. strain TY-5 prmB::Kanr | Gordonia sp. strain TY-5 with prmB disruption | 7 |
| E. coli DH5α | Host used for cloning | Takara Bio |
| Plasmids | ||
| pET21a | Vector used for cloning | Novagen |
| pETmimABCDgo | pET21a containing mimABCDgo of M. goodii 12523 | This study |
| pETmimABCDsm | pET21a containing mimABCDsm of M. smegmatis mc2155 | This study |
| pK18mobsacB | Vector used for deletion mutagenesis, aph sacB | NBRP (NIG, Japan) |
| pKΔmimA | pK18mobsacB containing a deleted mimA gene of M. smegmatis mc2155 | This study |
| pRHK1 | Rhodococcus (Mycobacterium)-E. coli shuttle vector, Kanr | 6 |
| pUCkap1 | pUC18 containing the kap1 promoter region from R. erythropolis | 13 |
| pRHKkap1 | pRHK1 containing the kap1 promoter region | This study |
| pRHKkap1mimAgo | pRHKkap1 containing the mimAgo gene under the control of the kap1 promoter | This study |
| pRHKkap1mimAsm | pRHKkap1 containing the mimAsm gene under the control of the kap1 promoter | This study |