FIG. 4.

Flow cytometric analyses of L. lactis NZ9000 cells expressing the fusion of B domain of staphylococcal protein A with AcmA peptidoglycan-binding domain (pSDLBA3b), the fusion of B domain with BmpA (pNZ::llmg_1064-B), and control cells (pNZ8148). The MFI is shown. Cells were stained with Alexa Fluor 488-conjugated rabbit anti-mouse antibody which bound to the B domain. Before staining with antibody, cells were washed with TBS (dark gray) or TBS+ (TBS containing 1 M NaCl and 1% SDS; light gray) as described in Materials and Methods. Vertical lines represent the standard deviations.