TABLE 1.
Antibacterial genes in metagenomic clonesa
| Clone, ORF name | No. of bp in ORF (no. of aa)/sig. pep. (position) | Transcrip. regions/positions | Cons. domain/position/E value/Pfam ID | % identity/% coverage | Amino acid length | Protein (Swiss-Prot accession no.) | Organism |
|---|---|---|---|---|---|---|---|
| CcAb1, abg1 | 1,245 (414)/yes (aa 31-32) | −10 box (GGTAATGAT)/bp 144; −35 box (TCTCCA)/bp 165 | GDSL-like lipase/aa 209-408/2.1 × 10−25/PF00657 | 26/13 | 806 | Minor extracellular protease vpr precursor (P29141) | Bacillus subtilis |
| 35/27 | 261 | Triosephosphate isomerase (A6WC54) | Kineococcus radiotolerans | ||||
| 31/31 | 382 | Subtilisin BPN precursor (P00782) | Bacillus amyloliquefaciens | ||||
| CcAb2, abg2 | 1,308 (435)/yes (1-26) | −10 box (CCATACAAT)/bp 146; −35 box (TTGCTT)/bp 174 | Beta-lactamase related/aa 48-429/2.7 × 10−67/PF00144 | 30/90 | 377 | Uncharacterized protein Rv1367c (Q11037) | Mycobacterium tuberculosis |
| 30/97 | 392 | Esterase estB (Q9KX40) | Burkholderia gladioli | ||||
| 24/92 | 434 | UPF0214 protein yfeW precursor (P77619) | Escherichia coli | ||||
| UaAb1, abg3 | 975 (325)/no (NA) | −10 box (AGCTATGCT)/bp 36; −35 box (TAGATA)/bp 56 | Abhydrolase_3/aa 88-291/4.6 × 10−59/PF07859 | 29/58 | 346 | Putative alpha/beta hydrolase R526 (Q5UQ83) | Acanthamoeba polyphaga mimivirus |
| 27/52 | 433 | Lipase 2 (P24484) | Moraxella sp. (strain TA144) | ||||
| 24/62 | 341 | AB hydrolase superfamily protein (Q9US38) | Schizosaccharomyces pombe |
Sig. pep. (position), the presence (yes) or absence (no) of the signal peptide and its respective position in the amino acid sequence; NA, not applicable; Transcrip. regions/positions, the presence of any transcriptional regions and their relative positions upstream of the antibacterial ORF; Cons. domain/position/E value/Pfam ID, the conserved domain identified from the Pfam database and its position in the amino acid sequence and the E value of a matched conserved domain and its respective Pfam identification number. Top hits from a protein similarity search of the antibacterial protein sequence against the curated part of the Swiss-Prot database, along with accession numbers, percentages of identity, percentages of coverage, lengths of the respective proteins, and the organism associated with the protein, are shown.