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. 2010 Dec 23;77(4):1187–1195. doi: 10.1128/AEM.02323-10

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FIG. 2. — HPLC analysis of the N-linked oligosaccharides of EGI and cell wall mannoproteins from wild-type (WT) and Ylmpo1Δ mutant strains. (A) N-linked oligosaccharides from the recombinant EGI secreted from wild-type (a), Yloch1Δ (b), Ylmpo1Δ (c), and Yloch1Δ Ylmpo1Δ (d) strains were labeled with PA and analyzed by NP-HPLC. (B) N-linked oligosaccharides from the CWPs of wild-type (a), Yloch1Δ (b), Ylmpo1Δ (c), and Yloch1Δ Ylmpo1Δ (d) strains were labeled with 2-AA and analyzed by NP-HPLC. The elution times of the peaks were compared with those of authentic PA- or AA-sugar chains (Man₇GlcNAc₂-AA and Man₈GlcNAc₂-AA) of known structure (indicated by arrows): M7, Man₇GlcNAc₂-PA; M8, Man₈GlcNAc₂-PA; M9, Man₉GlcNAc₂-PA; M10, Man₁₀GlcNAc₂-PA; 1*, monomannosylphosphorylated Man₈GlcNAc₂-PA; 2*, monomannosylphosphorylated Man₉GlcNAc₂-PA.