TABLE 1.
Oligonucleotides adopted in this work to obtain almost the entire 16S rRNA gene sequences of the symbionts for prevalence screenings and for FISH analysesa
| Target organism | Primer pair (sequence [5′-3′] or reference) |
Probe (fluorochrome-sequence [5′-3′] or reference) | |
|---|---|---|---|
| Forward | Reverse | ||
| “Ca. Phytoplasma solani” | PhF (CTAAACAGTTTTCATAGCATCACAA) | PhR (TTGTGATGCTATGAAAACTGTTTAG) | ph1107 (TR-GATGGCAATTAACAACAAGGGT) |
| Wolbachia | WF (TTAAATATGGGAAGTTTACTTTCTGTATTAC) | WR (GTAATACAGAAAGTAAACTTCCCATATTTAA) | W1 (27) |
| W2 (27) | |||
| “Ca. Cardinium hertigii” | EndoF1 (35) | EndoR3 (35) | card172 (Cy3-ATCTTTCTAGCATGCGCTAA) |
| card1069 (Cy3-GCACCTTGTATTCCGTCC) | |||
| “Ca. Sulcia muelleri” | SF (ATMTAGACAKAAAATATTCAGTG) | SR (CACTGAATATTTTMTGTCTAKAT) | S1150 (Cy3-ACATTCCAGTTACTCCTATCT) |
| SF1 (AGATAGGAGTAACTGGAATGT) | |||
| “Ca. Purcelliella pentastiridorum” | PF (GTATTTTATTAATAATAAAATATG) | PR (CATATTTTATTATTAATAAAATAC) | P820 (HEX/6-JOE/ROX-AGAAAACACGGCAAAATCACC) |
| PR1 (AGAAAACACGGCAAAATCACC) | |||
| HO1-V symbiont | VF (GATGAAGGTTGATAAGATC) | VR (GATCTTATCAACCTTCATC) | V370 (HEX/6-JOE/ROX-GATCTTATCAACCTTCATC) |
| VF1 (TTTTAAATTCTTTATAAAGTT) | |||
| Mollicutes | MCP52 (55) | ||
| Bacteroidetes | CFB319 (42) | ||
| Eubacteria | 27F (9) | 1495R (9) | EUB338 (4) |
| “Ca. Baumannia cicadellinicola” | Pro319 (42) | ||
a
The sequences of oligonucleotides designed and reported in previous studies are not shown.