TABLE 3.
Prevalence of symbionts in different organs or tissues of H. obsoletus determined with specific PCR assaysa
| Symbiont | No. of positive individuals/total no. of individuals testedb |
||||
|---|---|---|---|---|---|
| Whole insect | Gut | Ovaries | Testes | Salivary glands | |
| “Ca. Sulcia” | 60/70 (F, 28/31; M, 32/39) | 8/10 (F, 4/5; M, 3/5) | 4/5 | 2/5 | 2/10 (F, 1/5; M, 1/5) |
| Wolbachia | 43/70 (F, 23/31; M, 20/39) | 5/10 (F, 5/5; M, 0/5) | 3/5 | 0/5 | 2/10 (F, 2/5; M, 0/5) |
| “Ca. Cardinium” | 28/70 (F, 11/31; M, 17/39) | 3/10 (F, 2/5; M, 1/5) | 1/5 | 2/5 | 3/10 (F, 2/5; M, 1/5) |
| “Ca. Purcelliella” | 50/70 (F, 24/31; M, 26/39) | 3/10 (F, 2/5; M, 1/5) | 3/5 | 1/5 | 2/10 (F, 2/5, M, 0/5) |
| HO1-V | 61/70 (F, 28/31; M, 33/39) | 4/10 (F, 3/5; M, 1/5) | 4/5 | 1/5 | 3/10 (F, 1/5; M, 2/5) |
| “Ca. Phytoplasma solani” | 12/70 (F, 7/31; M, 5/39) | 2/10 (F, 1/10; M, 1/10) | 0/5 | 0/5 | 1/10 (F, 1/5; M, 0/5) |
a
A total of 80 individuals were used in the assays, including 36 females and 44 males. Seventy individuals were used as whole insects, while 10 were used for dissecting the different organs.
b
Number of individuals positive in specific PCR assays over the total number of tested individuals. M, males; F, females.