TABLE 1.
Primers used for nested RT-PCR assays
| Genogroup, region, and size (bp) | Primer/polarity | Sequence (5′-3′) | Positiona |
|---|---|---|---|
| Genogroup I | |||
| Capsid (241) | SRI-1/reverse | CCAACCCARCCATTRTACAT | 5652-5671 |
| SRI-2/forward | AAATGATGATGGCGTCTA | 5356-5373 | |
| SRI-3/reverse | AAAAYRTCACCGGGKGTAT | 5578-5596 | |
| Capsid (313) | NV-GIF1M/forward | CTGCCCGAATTYGTAAATGATGAT | 5342-5365 |
| NV-GIF2/forward | ATGATGATGGCGTCTAAGGACGC | 5358-5380 | |
| NV-GIR1M/reverse | CCAACCCARCCATTRTACATYTG | 5649-5671 | |
| Genogroup II | |||
| RdRp (203) | SRII-1/reverse | CGCCATCTTCATTCACAAA | 5078-5096 |
| SRII-2/forward | TWCTCYTTYTATGGTGATGATGA | 4583-4605 | |
| SRII-3/reverse | TTWCCAAACCAACCWGCTG | 4767-4785 | |
| Capsid (310) | NV-GIIF1M/forward | GGGAGGGCGATCGCAATCT | 5049-5067 |
| NV-GIIF3M/forward | TTGTGAATGAAGATGGCGTCGART | 5079-5102 | |
| NV-GIIR1M/reverse | CCRCCIGCATRICCRTTRTACAT | 5367-5389 |