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. 2010 Dec 23;77(4):1466–1474. doi: 10.1128/AEM.01996-10

TABLE 1.

Primers used for nested RT-PCR assays

Genogroup, region, and size (bp) Primer/polarity Sequence (5′-3′) Positiona
Genogroup I
    Capsid (241) SRI-1/reverse CCAACCCARCCATTRTACAT 5652-5671
SRI-2/forward AAATGATGATGGCGTCTA 5356-5373
SRI-3/reverse AAAAYRTCACCGGGKGTAT 5578-5596
    Capsid (313) NV-GIF1M/forward CTGCCCGAATTYGTAAATGATGAT 5342-5365
NV-GIF2/forward ATGATGATGGCGTCTAAGGACGC 5358-5380
NV-GIR1M/reverse CCAACCCARCCATTRTACATYTG 5649-5671
Genogroup II
    RdRp (203) SRII-1/reverse CGCCATCTTCATTCACAAA 5078-5096
SRII-2/forward TWCTCYTTYTATGGTGATGATGA 4583-4605
SRII-3/reverse TTWCCAAACCAACCWGCTG 4767-4785
    Capsid (310) NV-GIIF1M/forward GGGAGGGCGATCGCAATCT 5049-5067
NV-GIIF3M/forward TTGTGAATGAAGATGGCGTCGART 5079-5102
NV-GIIR1M/reverse CCRCCIGCATRICCRTTRTACAT 5367-5389
a

Norwalk virus was used for GI (GenBank accession number M87661), and Lordsdale virus was used for GII (GenBank accession number X86557).