FIG. 1.

Detail of the microfluidic device for on-chip staining and counting. (A) Design of microfluidic device. Long and short lengths are 50 mm and 25 mm, respectively. Width of the channel is 100 μm except for the “mixing part” (500 μm). Depth of the channel is 20 μm. Samples and fluorescent dye solution were injected at inlets A and B, respectively. Sheath fluid was injected at inlets C and D. (B) On-chip staining and counting of bacterial cells. (i) Samples and fluorescent dye solution flow separately and are mixed through the “mixing part” of the microchannel. (ii) Alignment of sample flow by sheath fluid. (iii) Flow of bacterial cells in the “detecting part” of the microchannel.