TABLE 5.

Habitat distributions of putative ecotypes demarcated by ecotype simulation and AdaptML

Gene	PE		No. of sequences from:		Habitat specificitye	P valuef
	Ecotype simulation	AdaptML	M60	M65
apcABb	1αg	A	20	35	M65	0.098
	1βg	Ba	4	0	M60
	2	C	0	3	M65
	3	D	2	0	M60
	4		1	0	M60
	5		1	0	M60
aroAc	1	A	52	12	M60	0.015
	2		2	0	M60
	4		1	0	M60
	3	B	0	3	M65
rbsKd	1	A	6	0	M60	<0.001
	2		4	0	M60
	3		1	0	M60
	4	B	1	1	M65
	5	C	1	2	M65
	6		1	3	M65
	7	D	1	1	M65
	8	E	3	1	M60
	9	F	0	7	M65
	10	G	17	5	M60
	11	H	0	5	M65
	12		1	5	M65
	13		0	5	M65
	14	I	0	1	M65

^aAdaptML ecotype B of apcAB contained the four sequences M60c639B6, M60c504C8, M60c096H4, and M60c494H7 from ecotype simulation PE1.

^bFor apcAB, ecotypes with an M60 habitat spectrum were estimated by AdaptML to occur 93% of the time at 60°C and 7% of the time at 65°C; ecotypes with an M65 habitat spectrum were estimated to occur 33% of the time at 60°C and 67% of the time at 65°C.

^cFor aroA, ecotypes with an M60 habitat spectrum were estimated to occur 79% of the time at 60°C and 21% of the time at 65°C; ecotypes with an M65 habitat spectrum were estimated to occur 22% of the time at 60°C and 78% of the time at 65°C.

^dFor rbsK, ecotypes with an M60 habitat spectrum were estimated to occur 83% of the time at 60°C and 17% of the time at 65°C; ecotypes with an M65 habitat spectrum were estimated to occur 13% of the time at 60°C and 87% of the time at 65°C.

^eIndicated by AdaptML.

^fFisher's exact test was employed as a contingency test for differences in habitat associations among ecotype simulation's putative ecotypes (only PE clades with >1 sequence were included).

^gPart of 1 PE.