TABLE 3.
Accumulation of transcripts in the central zone 2 and the peripheral zone 4 of microcolonies of A. nigera
| Zone (no. of hyphae) | Avg CT value (±SD) |
|||
|---|---|---|---|---|
| 18S rRNA gene | Actin gene | glaA | faeA | |
| Center (4,500) | 16.31 (±1.79) | 29.73 (±1.61) | 28.81 (±2.42) | 33.86 (±2.25) |
| Periphery (100) | 16.42 (±1.13) | 28.02 (±2.33) | 27.14 (±1.75) | 31.46 (±2.30) |
a
CT values were obtained with QPCR. QPCR was performed on the total RNA extracted from the 4,500 hyphae of zone 2 and the 100 hyphae of zone 4. Biological triplicates were used to calculate the average CT values and the standard deviations. glaA encodes glucoamylase, and faeA encodes ferulic acid esterase.