TABLE 1.
Genes related to sulfur metabolism differentially expressed in B. aurantiacum ATCC 9175 grown in the presence of cystine, sulfate, or methionine plus cystine or in the absence of any sulfur source
| Gene | Function and/or similarity | Ratio and P valuea |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| S/no sulfur |
S/MC |
C/MC |
S/C |
||||||
| Ratio | P | Ratio | P | Ratio | P | Ratio | P | ||
| BL146 | MetK, SAM synthase | 0.34 | 4.5E-6 | -* | 2.8E-2 | ||||
| BL613 | MetB, putative cystathionine γ-synthase | 0.26 | 2.8E-7 | 7.55 | 1.8E-4 | ||||
| BL1112 | CysE, serine acetyltransferase | 0.16 | 1.1E-12 | 2.82 | 2.1E-3 | 2.37 | 1.7E-2 | ||
| BL1113 | CysK, OAS thiol-lyase | 0.32 | 2.5E-5 | 8.65 | 1.2E-6 | 3.39 | 1.8E-5 | ||
| BL2145 | CysJ, sulfite reductase, flavoprotein | 0.38 | 6.1E-6 | 3.50 | 4.5E-2 | 2.86 | 2.8E-2 | ||
| BL2147 | Uroporphyrin III C-methyltransferase, C terminal | 0.47 | 2.9E-3 | 4.51 | 1.7E-3 | 2.47 | 8.5E-5 | ||
| BL2148 | CysN, sulfate adenylyltransferase | 0.33 | 5.1E-6 | 14.21 | 4.3E-5 | 3.53 | 2.41E-5 | ||
| BL2149 | CysD, sulfate adenylyltransferase | 0.44 | 2.5E-3 | 16.95 | 1.4E-7 | 3.38 | 3.1E-3 | ||
| BL2150 | CysH, phosphoadenosine 5′-phosphosulfate sulfotransferase | 0.26 | 4.5E-7 | 7.04 | 1.5E-3 | 2.79 | 6.1E-3 | ||
| BL2151 | CysI, sulfite reductase, hemoprotein | 0.25 | 3.0E-6 | 10.39 | 3.1E-3 | 3.58 | 7.4E-6 | ||
| BL2839 | MetE2, cobalamin-independent methionine synthase | 0.30 | 4.9E-7 | 4.02 | 2.2E-2 | ||||
| BL2146 | Unknown function | 0.46 | 7.1E-4 | 4.51 | 1.7E-3 | ||||
| BL1340 | MetE3, cobalamin-independent methionine synthase | 0.31 | 1.1E-6 | ||||||
| BL2496 | Protein sharing similarity with part of MetH | 0.27 | 9.0E-9 | ||||||
| BL1114 | Formylmethionine deformylase | 0.39 | 1.2E-5 | ||||||
| BL3618 | AecD, cystathionine β-lyase | 0.21 | 3.4E-10 | ||||||
| BL2478 | Glutamate-cysteine ligase | 0.18 | 8.0E-8 | ||||||
| BL3579 | IscS, cysteine desulfurase | 3.85 | 1.1E-7 | ||||||
| BL36 | MetX, homoserine acetyltransferase | 0.50 | 9.6E-3 | 5.26 | 7.4E-3 | -† | -‡ | ||
| BL56 | MetY, OAH thiol-lyase | -** | 1.7E-3 | 18.77 | 1.9E-4 | 16.13 | 8.0E-2 | 2.2 | 5.1E-2 |
| BL2266 | MetE1, cobalamin-independent methionine synthase | 16.10 | 0.0E+0 | 11.84 | 5.5E-10 | 32.40 | 5.5E-4 | 0.45 | 4.0E-2 |
| BL2267 | Unknown function | 19.3 | 0.0E+0 | 13.8 | 1.9E-10 | 32.4 | 3.5E-2 | ||
| BL891 | MerR-like regulator | 0.10 | 5.9E-06 | 0.07 | 3.6E-2 | ||||
| BL929 | Mgl, methionine γ-lyase | 0.06 | 5.5E-10 | 0.07 | 8.2E-2 | ||||
a
Abbreviations: S, sulfate; MC, methionine plus cystine; C, cystine. A P value of ≤0.05 was obtained using a Benjamini-Hochberg (BH) test under the S/no sulfur condition, and a P value of ≤0.1 was obtained using the BH test under S/MC, S/C, and C/MC conditions, where the difference is >2 or <0.5. *, BL146 = 1.8 under this condition; **, BL56 = 0.52 under this condition; †, based on qRT-PCR, the C/MC ratio is 7.0; ‡, based on qRT-PCR, the S/C ratio is 1.6.