TABLE 5.

Characterization of E. coli transformants with multidrug resistance plasmids from isolates of PFGE cluster VII

Transformant	PFGE pattern of donor	Antibiotic resistance patterna	Plasmid size (kb)	Target for PCRb
				intI	Class I integron (bp)	froR	SN-11	PU302-2	repFIIA	spvA	spvB	spvC	rck	traT	pefA
TF:TST205	VII-1	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT261	VII-2	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:R18-1	VII-3	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:07SY9	VII-4	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TFTST228	VII-5	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT262	VII-6	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT291	VII-7	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT302	VII-8	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:TST207-1	VII-9	AMP, CHL, STR, SUL,TET, CFZ	120	−	−	+	+	−	−	−	−	−	−	−	−
TF:TST207-4	VII-9	AMP, STR, SUL, TET, KAN	110	+	1,000	−	+	+	+	+	+	+	−	+	−
TF:KT165	VII-10	AMP, STR, SUL, TET, KAN	110	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:NST78	VII-11	AMP, STR, SUL, TET, KAN	110	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT158	VII-12	AMP, STR, SUL, TET, KAN	110	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT161	VII-13	AMP, STR, SUL, TET, KAN	110	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:NST110	VII-14	AMP, TET, KAN	97	−	−	−	−	+	+	+	+	+	+	+	−
TF:TST233	VII-15	AMP, STR, SUL, TET, KAN	120	+	1,000	−	+	−	+	+	+	−	+	+	−
TF:07IB1	VII-16	AMP, STR, SUL, TET, KAN	95	+	1,000	−	+	−	+	+	+	−	+	+	−
TF:HD	VII-18	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:TST31	VII-19	AMP, STR, SUL, TET, KAN	110	+	1,000	−	+	+	+	+	+	+	+	+	−
TF:KT271	VII-20	AMP, STR, SUL, TET, KAN	130	+	1,000	−	+	+	+	+	+	+	+	+	−

^aAMP, ampicillin; CHL, chloramphenicol; STR, streptomycin; SUL, sulfonamides; TET, tetracycline; KAN, kanamycin; CFZ, cefazolin.

^b+, presence of an amplification product from the primers directed at the indicated gene or fragment; −, absence of amplification product of PCR.