Normalized quantitative gene expression (relative to 16S rRNA) for heme copper oxidases (subunit Is include doxB, doxB-2, foxA, soxB, soxM), foxC, mco and tqoB genes. (A) Metallosphaera yellowstonensis grown on Fe(II), pyrite, S0, or YE. (B) Four high-temperature, acidic Fe(III) mats in Norris Geyser Basin (YNP). The inset shows a site photograph (Beowulf Spring) of one of three acidic geothermal systems sampled, indicating the location of geothermal discharge and one of the sampling locations (BED) discussed in this report. Gene expression bars without values were below the detection limit.