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. 2011 Jan 7;77(5):1674–1680. doi: 10.1128/AEM.01835-10

TABLE 1.

Strains and plasmids used in this study

Strain or plasmid Relevant characteristicsa Source or reference
Strains
    Candida boidinii Source of fdh gene Lab collection
    C. acetobutylicum
        DSM 1731 Wild-type strain DSMZ
        DSM 1731(pIMP1) Plasmid control strain of DSM 1731 harboring control vector pIMP1 This work
        DSM 1731(pITF) Recombinant strain of DSM 1731 harboring fdh expression vector pITF This work
    Escherichia coli
        TOP10 mcrA trio (mrr-hsdRMS-mcrBC) recA1 Invitrogen
        ER2275 recA mcrBC hsdR New England Biolabs
Plasmids
    pAN1 Cmr, Φ3T I gene, p15A origin 15
    pIMP1 MLSr Apr, shuttle vector of E. coli-C. acetobutylicum 15
    pITF fdh expression vector; the fdh gene from C. boidinii was ligated to pIMP1 This work
a

Cmr, chloramphenicol/thiamphenicol resistance; Apr, ampicillin resistance; MLSr, macrolide, lincosamide, and streptogramin B resistance; Φ3T I gene, Φ3T I methyltransferase gene of Bacillus subtilis phage Φ3T I.