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. 2011 Jan 14;77(5):1822–1832. doi: 10.1128/AEM.02501-10

FIG. 2.

FIG. 2.

Genetic determinants of Klus toxin. (A) Presence of L and M molecules in Klus strains. Nucleic acids were obtained from killer K1 (F166), K2 (EX73), K28 (F182), and Klus strains of different isotypes (Mlus-1 to Mlus-4) and separated by agarose gel electrophoresis. The ethidium bromide staining of the gel is shown. (B) Nuclease treatments. Nucleic acids from strains K2 and Klus-3 untreated, after DNase I digestion, or after RNase A treatment under high- or low-salt conditions were separated in an agarose gel. (C) Cycloheximide curing of killer viruses. Agarose gel electrophoresis of nucleic acids from killer K1 (F166), K2 (EX73), K28 (F182), and Klus strain EX229 before and after virus curing with cycloheximide (top); killer phenotype assay of the same strains (bottom). EX229-1 and EX229-2 are two cured clones from EX229. The assay was done on methylene blue agar plates (pH 4.7, 28°C) seeded with killer K2 (EX73) strain.