FIG. 6.

ORF expression from the Mlus 2nd AUG confers killer activity. (A) Secondary structure prediction of the 5′-terminal 57 nucleotides of Mlus positive strand using MFOLD (50). (B) Killer assay of strain 2927 transformed either with plasmid pRE1213 (containing the ORF, encoding the 242-amino acid protein, without the 5′-most-upstream AUG codon) or with the vector alone using 4.7 MB plates seeded with sensitive strain 5x47, with galactose as a carbon source to induce the expression of Klus toxin from the plasmid.