FIG. 4.

Effects of DNA concentration (A) and length of target sequence homology (B) on the frequency of lambda Red-mediated recombination in S. glossinidius. (A) Cultures of S. glossinidius were transformed with different amounts of a phoP::cat replacement allele that maintains 1,000 bp of target sequence homology flanking the genetic marker. (B) Cultures of S. glossinidius were transformed with fliM::cat replacement alleles maintaining different lengths of target sequence homology flanking the genetic marker. The frequency of recombination was found to increase in proportion to the length of target sequence homology. Transformations were carried out using 2 μg of each replacement allele. All optimization experiments were performed in triplicate; error bars represent standard deviations.