TABLE 1.
BLAST matches of free-living protozoan sequences obtained from DGGE bands
| Phylotype no. | Abbreviation | Closest relative(s) | GenBank accession no. | % similarity (no. of matching bp/total no. of bp) | Group |
|
|---|---|---|---|---|---|---|
| Taxonomic | Functional | |||||
| 16 | UROGLEN | Uroglena sp. CCMP2768 | EF165132 | 95.9 (140/146) | Chromalveolata | Flagellate |
| 19 | LOXOPH1 | Loxophyllum sp. GD-070419 | EU242511 | 97.1 (136/140) | Chromalveolata | Ciliate |
| 20 | LOXOPH2 | Loxophyllum spp.a | 97.6 (122/125) | Chromalveolata | Ciliate | |
| 21 | VAN | Vannella sp. CAZ6/1 | AY929914 | 90.8 (157/173) | Amoebozoa | Amoeba |
| 22 | PLATY | Platyamoeba placida | AY294150 | 92.8 (141/152) | Amoebozoa | Amoeba |
| 23 | ENCHELYS | Enchelys polynucleata | DQ411861 | 99.3 (141/142) | Chromalveolata | Ciliate |
| 25 | COLP | Colpoda spp.a | 98.1 (158/161) | Chromalveolata | Ciliate | |
| 26 | PARAPHYS | Paraphysomonas vestita | Z28335 | 86.8 (132/152) | Chromalveolata | Flagellate |
| 27 | GLISSOM | Glissomonad sp. Panama 103 | EU709272 | 98.1 (152/155) | Rhizaria | Flagellate |
| 29 | SPUMEL | Spumella-like flagellate JBM/S11 | EF043285 | 100 (146/146) | Chromalveolata | Flagellate |
| 30 | VORTICEL | Vorticella spp.a | 98.6 (145/147) | Chromalveolata | Ciliate | |
| 32 | SPUM/PAR | Spumella-like flagellate JBAS37 | AY651094 | 99.3 (149/150) | Chromalveolata | Flagellate |
| Paraphysomonas vestita | AF109325 | 99.3 (149/150) | ||||
| 34 | CYCL GL | Cyclidium glaucoma | EU032356 | 98.7 (155/157) | Chromalveolata | Ciliate |
| 35 | NAEGLE | Naegleria spp.a | 99.4 (159/160) | Excavata | Amoeba | |
| 37 | SPHAERO | Sphaeroeca WS 3-Uni05 | AJ867611 | 87.2 (136/156) | Opisthokonta | Flagellate |
| 48 | STRAMEN | Stramenopile E2-85 | EU528042 | 97.3 (142/146) | Chromalveolata | Flagellate |
| 58 | RHYN NAS | Rhynchomonas nasuta HFCC348 | DQ46552 | 100 (166/166) | Excavata | Flagellate |
a
Several species within one genus all gave the same similarity results.