TABLE 1.

Kinetic characteristics of DatA with six halogenated substrates^a,b

Substrate	K0.5 (mM)	kcat (s−1)	kcat/K0.5 (s−1·mM−1)	nHe	mHf	Ksi (mM)
1,3-Dibromopropanec	2.15 ± 0.15	1.47 ± 0.08	0.69	1.0	—g	—
1,3-Dichloropropanec	>4	>0.008	< 0.002	1.0	—	—
1,3-Diiodopropanec	0.20 ± 0.03	1.2 ± 0.1	6.0	3.5 ± 1.6	—	—
1-Bromohexanec	0.14 ± 0.09	1.7 ± 0.1	12.20	1.0	—	—
1-Chlorohexanec	0.07 ± 0.02	0.039 ± 0.004	0.58	1.27 ± 0.23	—	—
1-Iodohexaned	0.63 ± 0.25	0.26 ± 0.09	0.42	2.4 ± 1.0	11.6 ± 6.0	1.7 ± 0.1

^aAll measurements were performed in 100 mM glycine buffer with pH 8.6 at 37°C.

^bPlus or minus standard error of the fit.

^cAll parameters were calculated using the Hill equation: (where S is the substrate concentration and v_lim is the limiting rate at the saturating substrate concentration).

^dAll parameters were calculated using the Hill equation with substrate inhibition: .

^eHill coefficient.

^fHill coefficient in inhibitory mode.

^g—, not applicable for the scheme used in a fit.