TABLE 5.
Effect of maize kernel washing treatments on competitive advantage conferred to the first A. flavus isolate to contact maize kernels
| Kernel washb | Inoculum suspensionc | % YV04 DNA (SE)a |
|
|---|---|---|---|
| Kernels | Conidia | ||
| Tween 80 | Water | 81 (3) a | 78 (2) a |
| Water | Water | 77 (2) ab | 68 (3) b |
| None | Water | 74 (3) b | 69 (2) b |
| Hexane | Water | 75 (2) b | 67 (2) b |
| Ethanol | Water | 70 (2) b | 66 (3) b |
| None | Tween 80 | 72 (4) b | 65 (3) b |
Proportion of total A. flavus DNA comprised of that of YV04. Isolate YV04 was inoculated 1 h prior to inoculation of CG136. A. flavus DNA from kernel-infecting hyphae and conidia from kernel surfaces were analyzed separately. Means followed by the same letter are not significantly different by Fisher's LSD test.
Kernels were submerged in each of the designated solutions and washed by gentle agitation. “None” indicates kernels were not subjected to a washing treatment prior to inoculation.
Conidial inoculum for each isolate was suspended in either water or 1% Tween 80.