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. 2010 Dec 30;77(5):1862–1871. doi: 10.1128/AEM.01918-10

FIG. 7.

FIG. 7.

HPP does not degrade viral capsid proteins. (A) Visualization of MNV-1 capsid protein by 12% SDS-PAGE. The purified MNV-1 was pressurized at 350, 500, and 600 MPa at 4°C for 2 min. Total viral proteins were analyzed by 12% SDS-PAGE followed by Coomassie staining. VP1, major capsid protein. (B) Visualization of MNV-1 capsid protein by 15% SDS-PAGE. The purified MNV-1 was pressurized at 600 MPa at 4°C for 2 min. Total viral proteins were analyzed by 15% SDS-PAGE followed by Coomassie staining. VP2, minor capsid protein. (C) Western blot analysis of VP1 protein. Identical samples from panel B were separated by SDS-PAGE and subjected to Western blotting using rabbit anti-MNV VP1 polyclonal antibody.