FIG. 1.

Production of scFv anti-SAI/II by L. paracasei transformed with plasmids containing different expression cassettes. (A) The APF protein can be divided in three domains, N-terminal (N-ter), C-terminal (C-ter), and a central region which is rich in asparagine, glutamine, threonine, and alanine. The promoter (P), the ribosomal binding sites (RBS), the signal peptide (sp), the translational stop codon (arrowhead), and the transcription terminator (lollipop) are indicated. (B) scFv production was analyzed in cell extract (c) and supernatant (s) of L. paracasei transformed with the plasmids pAF100 to pAF1100. The experiment was repeated twice, and all transformants were analyzed at the same time. An equivalent of 125 μl supernatant and extract from 1 × 10⁸ cells was loaded in each well.