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. 2011 Jan 21;77(6):2174–2179. doi: 10.1128/AEM.02690-10

FIG. 2.

FIG. 2.

Evaluation of surface display of scFv anti-SAI/II in modified L. paracasei. (A) Demonstration of noncovalent attachment of scFv to the surface of L. paracasei pAF400 by Western blotting. (i) The bacterial pellet was treated with LiCl (5 M) to strip surface proteins, and Western blotting of the cell extract was performed. (ii) Cell extract from wild-type L. paracasei incubated with the culture supernatant of wild-type L. paracasei (wt), L. paracasei pAF100, or L. paracasei pAF400 to evaluate reattachment of scFv. (B) Flow cytometry analysis showing the display of antibody fragments by L. paracasei transformants displaying surface-anchored scFv anti-SAI/II antibody fragments.