TABLE 1.

Most important strains and plasmids used in this study

Strain or plasmid	Relevant properties	Reference or source
Strains
E. coli DH5α
L. paracasei	Previously considered a plasmid-free L. casei 393	15
L. paracasei pAF100	L. paracasei with pAF100 plasmid, secreted scFv anti-SAI/II	This work
L. paracasei pAF400	L. paracasei with pAF400 plasmid, secreted and attached scFv anti-SAI/II	This work
L. paracasei pAF900	L. paracasei with pAF900 plasmid, surface-anchored scFv anti-SAI/II	This work
L. paracasei pAF900-ARP1	L. paracasei with pAF900-ARP1 plasmid, surface-anchored ARP1	This work
L. paracasei EM171	L. paracasei with integrated pAF400 cassette, secreted and attached scFv anti-SAI/II	This work
L. paracasei EM181	L. paracasei with integrated pAF900 cassette, surface-anchored scFv anti-SAI/II	This work
L. paracasei EM182	L. paracasei with integrated pAF100 cassette, secreted scFv anti-SAI/II	This work
L. paracasei EM233	L. paracasei with integrated pAF900-ARP1 cassette, surface-anchored ARP1	This work
Plasmids
pIAV7	Broad-range vector, Err, lacZ, pWV01 replication origin	16
pAF100 to pAF1100 series	pIAV7 with 11 expression cassettes	This work
pEM76	Integrative vector containing six1, A2 int, attP, and six2	12
pEM94	Containing the β-recombinase gene in order to delete the non-food-grade DNA present in the integrated plasmids by site-specific recombination	13
pEM171	pEM76 with expression cassette of pAF400	This work
pEM181	pEM76 with expression cassette of pAF900	This work
pEM182	pEM76 with expression cassette of pAF100	This work
pEM233	pEM76 with expression cassette of pAF900-ARP1	This work