FIG. 2.
Effect of O2 on A. macleodii hydrogenase abundance and its activity in AltDE. (A) Examination of H2 evolution activity in crude cellular extracts from AltDE cells. The AltDE cells were grown in marine broth medium for 18 h under aerobic conditions (+O2) or incubated under anaerobic conditions (−O2). The bars represent average values ± the standard deviations (SD) of three independent experiments. (B) Immunodetection of HynL in crude cellular extract of AltDE after native PAGE. Crude extracts from the panel A were loaded in the gel, and protein blots were probed with anti-TrHynL antibodies. Crude extract from T. roseopersicina GB2131 was loaded as a positive control. (C) SDS-PAGE gel stained with Coomassie brilliant blue. Portions (40 μg) of total protein were loaded into each lane for Western blotting and Coomassie blue staining.