TABLE 2.
Oligonucleotide primers used in this study
| Name | Sequence | Relevant description |
|---|---|---|
| LuxSF | CCTTGAATTCAGGATGCCGTTGTTAGATAGC | Upstream primer for cloning luxS from W3110 |
| LuxSR | AACTGAATTCCGGCTAGATGTGCAGTT | Downstream primer for cloning luxS from W3110 |
| RTLuxSF | GATGCCGTTGTTAGATAGCTTCAC | Upstream primer for luxS RT-PCR |
| RTLuxSR | CTAGATGTGCAGTTCCTGCAAC | Upstream primer for luxS RT-PCR |
| pkk223LuxSF | ACGCATATGTCCTACTCAGGAGAGCGTTCA | Downstream primer for cloning tac promoter-luxS fusion from pKKluxS |
| pkk223LuxSR | AGCCATATGTCGCTCAAGGCGCACTCCCG | Downstream primer for cloning tac promoter-luxS fusion from pKKluxS |
| LacIqF | GGAGCTGCATGTGTCAGAGGTT | Upstream primer for cloning lacIq from pTrcHisB |
| LacIqR | CAAAAAACATTATCCAGAACGGGAG | Downstream primer for cloning lacIq from pTrcHisB |
| FCAT | TAAAAGACATGTGGGGTTCTCATCATCATC | Upstream primer for cloning cat and the GFPuv gene from pTrcHisCAT and pTrcHisGFPuv, respectively |
| RCAT2 | TTAATGTTTAGCGGCCGCTTAAAAAAATTACGC | Downstream primer for cloning cat from pTrcHisCAT |
| RGFPuv | TTAATGTTTAGCGGCCGCCAGCTTTCATTATTT | Downstream primer for cloning the GFPuv gene from pTrcHisGFPuv |
| RTgroELF | GGCAGCTAAAGACGTAAAATTCGG | Upstream primer for groEL RT-PCR |
| RTgroELR | CATGCATTCGGTGGTGATCATC | Downstream primer for groEL RT-PCR |
| RTdnaKF | GGGTAAAATAATTGGTATCGACCTGGG | Upstream primer for dnaK RT-PCR |
| RTdnaKR | GTCTTTGACTTCTTCAAATTCAGCGTC | Downstream primer for groEL RT-PCR |
| 16S-2F | AGCGCAACCCTTATCCTTTGTTGG | Upstream primer for 16S rRNA RT-PCR internal control |
| 16S-2R | TCGCGAGGTCGCTTCTCTTTGTAT | Downstream primer for 16S rRNA RT-PCR internal control |