TABLE 1.
Oligonucleotide sequences for real-time SNP amplification
| SNP | Sequencea |
|||
|---|---|---|---|---|
| Forward primer | Reverse primer | Probe 1 (5′-VIC/3′-NFQ-MGB) | Probe 2 (5′-FAM/3′-NFQ-MGB) | |
| 769 | GCA-ACA-CAA-TGC-CAT-GAA-CGA-ATT | AAT-TGA-ATA-TCG-TCA-ATT-AAC-AAC-GCA-TTT | AGA-GCG-GTA-AAA-GC | AAG-AGC-GAT-AAA-AGC |
| 2287 | GCG-TTT-ACT-CGA-GGA-GGA-TGA-AG | GAA-ACA-CCG-ATG-TGA-TTA-TTC-CCA-ATT-C | CTG-GCC-GCT-ATC-C | CTG-GCC-ACT-ATC-C |
| 4439 | TCT-GCC-GCC-GAA-GTT-ATT-ATG-AC | CCG-CCG-GAA-ACT-TTA-TAA-GCA-TTT-T | CTT-CAC-GCA-GGC-GGT | CTT-CAC-GCC-GGC-GGT |
| 4557 | ATC-AGA-AGA-ACT-CTA-TAT-GAC-TAT-TCG-C | GAC-GCC-CAT-TTT-GTA-GGT-TTG-A | CAA-AAC-GGT-AAC-ATC-TAT-AC | AAA-CGG-TAA-CGT-CTA-TAC |
| 4844 | CGG-TAT-AAA-AGC-TTT-CGT-TGA-ACA-TTT | CTT-TTC-AGT-GGA-AAA-ATG-GAA-GAC-GT | AAA-CCC-CCA-TTC-ATT-CA | AAC-CCC-CAT-CCA-TTC-A |
| 5423 | TTC-CTG-GTG-GAA-GGC-GAT-TC | GGC-GAT-CAC-GGG-CTT-GT | TAG-CCG-AAC-CAC-CAG-CC | CCG-AAC-CGC-CAG-CC |
| 6025 | TTG-CAG-ATT-TGA-GTG-AGG-AGG-AAA-A | GTT-TTG-GGT-TCG-TTT-AAC-TGT-TCC-T | CAA-CCG-CTG-TCT-CCA-AG | ACC-GCT-GTG-TCC-AAG |
| 7087 | CTT-CCC-GCG-CCT-CGA-T | GCG-CTT-GAA-CGT-CTT-GTT-GTT | CAC-CGT-TTT-ACC-CTG-CAC-AA | CCG-TTT-TAC-CCC-GCA-CAA |
| 7726 | CTT-GTG-TGG-CGT-TAG-CGA-AT | ACG-TTT-CTT-GGC-GTG-TTA-AAA-GC | AAA-ATG-CGC-CGA-TCA-T | AAT-GCG-CGG-ATC-AT |
| 7974 | CAT-AAT-TCA-TCA-AGG-CAC-CAA-TGG-T | TGA-AAA-GAA-AGC-GGT-TGC-ATT-CG | CGG-CAT-CAC-AAT-TTA | CGG-CAT-CAC-GAT-TTA |
a
The underlined nucleotides represent the location of the SNP.