TABLE 2.
CAT specific activities representing the expression levels of PfruA-cat and PlevD-cat fusions in the wild-type background growing on various carbohydrates
| Fusion | CAT activitya (SD) in cells grown on the indicated carbohydrate |
||||||
|---|---|---|---|---|---|---|---|
| Glc | Fru | Gal | Inulin | Glc/inulin | Fru/inulin | Gal/inulin | |
| PfruA-cat | 0.0 (0.0) | 0.0 (0.0) | 0.0 (0.0) | 24.8 (1.6) | 7.5 (1.0) | 0.0 (0.0) | 9.5 (0.4) |
| PlevD-cat | 14.9 (1.9) | 8.9 (2.4) | 21.0 (5.7) | 630.1 (27) | 235.6 (19) | 12.9 (2.4) | 338.7 (49) |
a
CAT activity is expressed as nmol of chloramphenicol acetylated mg of protein−1 min−1, followed by the standard deviation in parentheses. The data are averages from at least three independent cultures, and all assays were performed in triplicate. The carbohydrate concentration in the growth medium was 0.5% (wt/vol). Glc, glucose; Fru, fructose; Gal, galactose.