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Bacterial challenge assay with SPC35 to S. Typhimurium SL1344 (A) and E. coli MG1655 (B). SPC35 (100 μl) was added at an MOI of 0.1 (open circles), an MOI of 1 (open squares), or an MOI of 10 (closed circles) to the bacterial culture after 1.5 h incubation (vertical arrows, OD600 = 0.5 to 0.6). A bacterial culture inoculated with SM buffer instead of SPC35 was used as the negative control (closed squares).
