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. 2010 Dec 22;18(2):210–216. doi: 10.1128/CVI.00296-10

FIG. 4.

FIG. 4.

Platelet cytokine secretion in response to IgG-coated targets. Platelets were stimulated with thrombin (20 U/ml) or 1.5-μm polystyrene beads (10 beads per platelet) coated with BSA or IgG and assessed for secretion of sCD40L and RANTES by ELISA. Other samples were pretreated with 5 μg/ml anti-FcγRIIA MAb or 5 μg/ml isotype-matched control IgG to assess the role of FcγRIIA. Other samples were pretreated with 20 μM cytochalasin D (Cyto D) to determine the role of actin rearrangement in cytokine secretion. Data are means ± SD representative of one experiment performed in triplicate. Experiments were repeated on 5 different days with platelets from five healthy volunteers with similar results. *, P < 0.01; **, P < 0.001.