Percentages of CD26+ T cells among PBMCs isolated from control noninfected calves and calves infected with Mycobacterium avium subsp. paratuberculosis by the following methods: oral, intraperitoneal, oral with dexamethasone administration, and oral with mucosal scrapings from a cow with clinical disease. Percentages of CD26+ T cells during the 12-month study were measured within CD4 (A), CD8 (B), and γδ (C) T-cell subpopulations stimulated with a whole-cell sonicate of Mycobacterium avium subsp. paratuberculosis (MPS). Data are expressed as means ± SEM. Significant differences between control and infection groups within given time points are represented by asterisks (**, P < 0.01; *, P < 0.05).