TABLE 1.
Primary antibodies used for this studya
| Antigen | MAb clone | Isotype | Working MAb concnb (μg/ml) | Specificity |
|---|---|---|---|---|
| CD4 | GC50A1 | IgM | 14 | T-helper cell |
| CD5 | B29a | IgG2a | 7 | Activation marker |
| CD8 | BAQ111A | IgM | 14 | T-cytotoxic/suppressor cell |
| CD25 | CACT116A | IgG1 | 15 | IL-2 receptor |
| CACT108A | IgG2a | 15 | ||
| LCTB2A | IgG3 | 15 | ||
| CD26 | CACT114A | IgG2b | 15 | Activation marker |
| CD45RO | GC42A1 | IgG1 | 10 | Memory/activation marker |
| N12 | CACT61A | IgM | 14 | γδ-Cell receptor |
a
Obtained from VMRD Inc. (Pullman, WA).
b
Diluted in PBS with 1% fetal calf serum and 0.04% sodium azide.