TABLE 1.
Analyte | Microsphere region | Capture antibody | Detection antibody | Standard |
---|---|---|---|---|
Feline IFN-γ | 15 | Goat anti-feline IFN-γ | Biotinylated goat anti-feline IFN-γ | Recombinant feline IFN-γ |
Feline IL-10 | 33 | Mouse anti-feline IL-10 | Biotinylated goat anti-feline IL-10 | Recombinant feline IL-10 |
Feline IL-12/IL-23 p40 | 55 | Goat anti-feline IL-12/IL-23 p40 | Biotinylated goat anti-feline IL-12/IL-23 p40 | Recombinant feline IL-12/IL-23 p40 |
The analyte, capture antibody, detection antibody and standard were from the DuoSet ELISA (R&D Systems). The microsphere region (MagPlex-C microspheres; Luminex) was assigned by the manufacturer based on the ratio of two internal dyes.