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Optimized concentrations of reagents
| Cytokine | Capture antibody (μg/106 microspheres) | Detection antibody (ng/ml) | Streptavidin-PE (μg/ml) | Microsphere input (per region per well) |
|---|---|---|---|---|
| IFN-γ | 5 | 2,400 | 3 | 2,500 |
| IL-10 | 5 | 200 | 3 | 2,500 |
| IL-12/IL-23 p40 | 5 | 300 | 3 | 2,500 |
