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IFN-γ concentration determined in culture supernatants of stimulated PBMCs. Culture supernatants of the peripheral blood mononuclear cells from immunized animals were tested by ELISA. Data represent means ± standard deviations. (A) Culture supernatants of protein-stimulated or mock-stimulated PBMCs; (B) culture supernatants of DEN-2-stimulated PBMCs or mock-stimulated PBMCs. These experiments were repeated three times, with the same results achieved each time (n = 3).
