Fig. 6.

rad52Δ cells undergo TRD that often has appreciable turnover associated with it. (A) Southern blot showing XhoI digest of two TRD events in a rad52Δ strain containing the long Bcl telomere. Lane WT, URA3-tagged telomere that is wild type in length and sequence; lane P, precursor URA3-tagged long Bcl telomere from immediately prior to isolation of the subclones in the other lanes. The long Bcl telomere in the precursor was slightly longer than the same telomere in the subclones because of gradual sequence attrition in the latter from undergoing more cell divisions. (B) Southern blot showing XhoI digest of an apparent slight elongation event in a rad52Δ strain. Lanes marked WT and P indicate wild-type and precursor Bcl telomeres, as described for panel A. (C) Southern blot of a 1.5% agarose gel showing XhoI and XhoI-plus-BclI digests of two subclones that had undergone TRD to near-wild-type size in a rad52Δ strain. The position of the leftover wild-type block of repeats is shown with an arrow. Markers in panels A and B are shown in kilobases, and markers in panel C are shown in base pairs.