Table 1.

Molecular features of fungal Skn7 orthologs

Organism	Protein	Protein size (aa)	Distance (aa) from N terminus to HSFc	% identityb to HSF domain (135 aa)	Distance (aa) from HSF to RRd	% identity to RR domain (125 aa)	Probability score for CC domaine
Animal pathogens
C. glabrata	CgSkn7	630	73	71	228	92	0.85
C. albicans	CaSkn7	559	24	58	296	75	0.999
C. neoformans	CnSkn7	1,039/1,040a	202	47	384	50	0.989
P. marneffei	PmSkn7	614	13	57	243	47	0.995
C. lusitaniae	ClSkn7	478	10	61	185	75	0.997
A. fumigatus	AfSkn7	622	15	56	246	47	0.997
Plant pathogens
C. heterostrophus	Dic2/ChSkn7	635	1f	52	244	47	0.990
M. oryzae	MoSkn7	671	9g	53	244	46	0.53
Model organisms
A. nidulans	SrrA/AnSkn7	558	1f	60	240	47	0.895
S. pombe	Prr1/SpSkn7	539	6	60	260	42	0.081
N. crassa	Rrg2/NcSkn7	661	16	58	172	46	0.929
S. cerevisiae	ScSkn7	622	83	100	190	100	0.55

^aThere is a slight size difference between the Skn7 proteins in C. neoformans var. grubii and C. neoformans var. neoformans.

^bRelative to ScSkn7.

^cHSF, heat shock factor-like DNA binding domain.

^dRR, response regulator domain.

^eHighest probability score for a coil-coiled region (CC domain) within 50 bp of the HSF domain (http://www.ch.embnet.org/software/COILS_form.html). Parameters: MTIDK matrix, positions a and d weighted, window width of 21.

^fPosition 1 of the sequence aligns with position 11 in the alignment.

^gTrue alignment is between position 23 of the M. oryzae sequence and position 50 (helix α2) of the alignment.