TABLE 1.
Primers used for the construction of overexpression strains and the sigB deletion strain
| Primera | Sequenceb |
|---|---|
| dxs-BamHI-F | CGGGATCCTTGGATCTTTTATCAATACAGGAC |
| dxs-XbaI-R | GCTCTAGATCATGATCCAATTCCTTTGTG |
| dxr-BamHI-F | CGGGATCCTTGAAAAATATTTGTCTTTTAGGAGCA |
| dxr-XbaI-R | GCTCTAGA TCA CGA ACA TAC CAC CTT ATG |
| dxs-SOE-R | TCA TGA TCC AAT TCC TTT GTG |
| dxr-SOE-F | CAC AAA GGA ATT GGA TCA TGA TTG AAA AAT ATT TGT CTT TTA GGA GCA |
| sigB-BamHI-A1-F | CGGGATCCGCGGTTCAGCACGCTTACAAAGAA |
| sigB-B1-R | GCGTTTCCTGCGCTTGTTCATCTT |
| sigB-C1-F | AAGATGAACAAGCGCAGGAAACGCGGGACATTCTCGGTATATCTCAAATGCACG |
| sigB-XbaI-D1-R | GCTCTAGATTTCAGGATAGATGCTGTAGCGCC |
a
F, forward primer; R, reverse primer.
b
Primers were designed using published sequences available at the GenoList server (http://genodb.pasteur.fr/cgi-bin/WebObjects/GenoList). The restriction sites incorporated into primers for cloning purposes are shown in boldface and underlined. The overhangs complementary to the dxs-SOE-R primer and the sigB-B1-R primer are underlined in the sequences of primers dxr-SOE-F and sigB-C1-F, respectively.