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Primers used for real-time RT-PCR
| Gene targeted | Primer orientationa | Primer sequenceb |
|---|---|---|
| 16S rRNA gene | F | ACGGTCGCAAGACTGAAACT |
| R | TAAGGTTCTTCGCGTTGCTT | |
| dxs | F | GAAAATGAAGGCCAGCACAT |
| R | CATCCATTTTTACGCCGAGT | |
| dxr | F | GCGCTTGCAAATAAGGAAAC |
| R | GGCTGAATGCTCACTGTCAA | |
| sigB | F | GCCGAAAGTCGAAGAGATTG |
| R | GGTCAACGGATAAGGCTTGA |
F, forward primer; R, reverse primer.
Primers were designed using published sequences available at the GenoList server (http://genodb.pasteur.fr/cgi-bin/WebObjects/GenoList).
