TABLE 2.
Key oligonucleotide primers used in this study
| Primer | DNA sequence (5′-3′)a | Product size (bp)b | Gene amplifiedb |
|---|---|---|---|
| Cj1125cF | GCCCGCTAGAATGTCTTTGA | 1,733 | Cj1125c |
| Cj1125cR | ATCTAACCCGGGACGATTTT | ||
| Cj1116cF | GGAACTCATTGATGAAATGCAA | 1,982 | Cj1116c |
| Cj1116cR | CCCTACCATCTATAGGTGCAAAA | ||
| CmAfeI-F | GCGAGCGCTTGCTCGGCGGTGTTCCTTT | 811 | cat |
| CmAfeI-R | GCGAGCGCTGCGCCCTTTAGTTCCTAAAG | ||
| Cj1687F1 | TCTTTGGCATCTTTGGCTTT | 2,000 | Cj1687 |
| Cj1687R1 | TGCGATTTTGATGTTTCC | ||
| Cj0630cF | CAACGAAAAACAAAGCAA | 1,350 | Cj0630c |
| Cj0630cR | TGTTTTTAAGTTCTTCGATTTTTGC | ||
| PerRF2 | AAACAAGTAAGGTGGAA | 1,662 | Cj0032 (perR) |
| PerRR2 | AGTGCAATCAGATAGTAAA |
a
The underlined and italic sections within the primer sequences represent the AfeI restriction sites.
b
Product sizes and amplified genes refer to those of the relevant primer pairs.