TABLE 5.
Differentially expressed genes (≥2-fold changes) in NCTC 11168 OR-7r mutant JL341 identified by DNA microarray and real-time qRT-PCR
| Gene | Function categories and description of product | P value | Fold change by microarray | Fold change by qRT-PCRa |
|---|---|---|---|---|
| Upregulated genes | ||||
| Cj0366c (cmeB) | Inner membrane transporter of CmeABC efflux system | 5.93E−6 | 4.9 | 6.7 |
| Cj0365c (cmeC) | Outer membrane component of CmeABC efflux system | 2.72E−5 | 4.0 | 7.4 |
| Cj0630c | DNA polymerase III, delta subunit | 4.63E−4 | 13.5 | ND |
| Cj1726c | Putative homoserine O-succinyltransferase | 8.31E−4 | 2.0 | ND |
| Cj1116c | Putative membrane-bound zinc metallopeptidase | 4.00E−3 | 4.8 | 4.0 |
| Cj1125c | Putative galactosyltransferase (WlaG/PglA) | 5.50E−3 | 12.8 | 8.0 |
| Cj0176c | Putative lipoprotein | 7.05E−3 | 2.0 | ND |
| Cj1687 | Putative efflux transporter (MFS family) | 2.14E−2 | 2.0 | 2.0 |
| Cj0035c | Putative efflux transporter (MFS family) | 2.19E−2 | 3.4 | 2.0 |
| Downregulated genes | ||||
| Cj0508 | Penicillin-binding protein | 2.37E−5 | −4.5 | ND |
| Cj0093 | Putative periplasmic protein | 1.49E−4 | −2.5 | ND |
| Cj0628 | Putative lipoprotein | 1.57E−4 | −2.9 | ND |
| Cj0045c | Putative iron-binding protein | 6.60E−4 | −2.0 | ND |
| Cj0091 | Putative lipoprotein | 8.90E−4 | −2.5 | ND |
| Cj0629 | Putative lipoprotein | 1.16E−3 | −2.7 | ND |
| Cj1423c | Putative sugar-phosphate nucleotidyltransferase | 1.49E−3 | −2.7 | ND |
| Cj1650 | Hypothetical protein | 1.80E−3 | −17.9 | ND |
| Cj1714 | Small hydrophobic protein | 4.56E−3 | −2.4 | ND |
| Cj1539c | Putative anion-uptake ABC transport system protein | 9.39E−3 | −2.1 | ND |
a
Means of three independent experiments. ND, not determined.