FIG. 5.

Vader reintegration. Gel electrophoresis of three different TAIL-PCR products from excision events E1, E2, and E3. Lane designations R1, R2, and R3 indicate products of the primary, secondary, and tertiary TAIL-PCRs, respectively. The random oligonucleotide IB1347 and nested oligonucleotides IB1345, EH1434, and IB1336 were employed. The white arrows indicate PCR fragments which were sequenced and found to contain A. niger genomic sequences flanking the transposon Vader at its reintegration site.