TABLE 1.

Thermal cycling conditions used for primary, secondary, and tertiary TAIL-PCR

TAIL-PCR	File no.	No. of cycles	Thermal setting(s) (time)
Primary	1	1	92°C (2 min), 95°C (1 min)
	2	4 high-stringency cycles	94°C (15 s), 55°C (1 min), 72°C (2 min)
	3	1 low-stringency cycle	94°C (15 s), 30°C (3 min), 30°C (+ 0.2°C/s)
			18°C (+ 0.3°C/s), 72°C (2 min)
	4	9 reduced-stringency cycles	94°C (5 s), 37°C (1 min), 72°C (2 min)
	5	11 super cycles	94°C (5 s), 55°C (1 min), 72°C (2 min)
			94°C (5 s), 55°C (1 min), 72°C (2 min)
			94°C (5 s), 37°C (1 min), 72°C (2 min)
	6	1	72°C (5 min)
Secondary	7	1	94°C (2 min)
	8	1	94°C (5 s), 55°C (1 min), 72°C (2 min)
	9	9 super cycles	94°C (5 s), 55°C (1 min), 72°C (2 min)
			94°C (5 s), 55°C (1 min), 72°C (2 min)
			94°C (5 s), 37°C (1 min), 72°C (2 min)
	6	1	72°C (5 min)
Tertiary	10	1	94°C (2 min)
	11	19 normal cycles	94°C (30 s), 37°C (1 min), 72°C (2 min)
	6	1	72°C (5 min)