TABLE 1.

Summary of catabolized carbohydrate and excreted products after 4 days of autofermentation

Category	Value (mol × 1017 cells−1) for:
	Intracellular R.S.C.a	Hydrogen excreted	Acetate excreted	Lactate excreted	Alanine excreted	CO2 excretedc	Excess equivalents excretedd
WT cells (measured)	31 ± 14	2.9 ± 0.3	6.5 ± 0.4	60 ± 7	1.8 ± 0.8	6.5
nifJ cells (measured)	26 ± 5	2.3 ± 0.2	3.0 ± 0.4	51 ± 7	3.0 ± 1.0	3
Carbon equivalents per moleculeb	−6	0	2	3	3	1
NADH equivalents per moleculeb	−2	1	−1	1	1	0
WT carbon equivalents	−186	0	13	180	5.4	6.5	18.9
nifJ carbon equivalents	−156	0	6	153	9	3	15
WT NADH equivalents	−62	2.9	−6.5	60	1.8	0	−3.8
nifJ NADH equivalents	−52	2.3	−3	51	3	0	1.3

^aR.S.C., reduced sugars catabolized.

^bValues represent theoretical equivalents of carbon (row 3) and reductant (row 4 [as NADH]) per catabolized glucose equivalent during fermentation.

^cCO₂ values were not directly measured and are set equal to the acetate concentration, since CO₂ forms stoichiometrically and concomitantly with acetyl-CoA via the PFOR or PDC reaction (Fig. 1). Additionally, the reductant formed in this reaction is accounted for in the acetate column only to avoid double counting.

^dExcreted equivalents that were excreted in excess of the carbohydrate catabolized. Based on the systematic error determined for each detection method, the propagated systematic error for this column is ±15 for carbon equivalents (rows 5 and 6) and ±5 for NADH equivalents (rows 7 and 8) based on the systematic error for each detection method.