FIG. 4.

Protein degradation by strains OSP1675 and OSP1689 in solidified and liquid media. (A) Formation of halos in solid media containing 3.5% skim milk. Cell suspensions were inoculated as described in the text and incubated at 85°C for 3 days. (B and C) Degradation of bovine serum albumin (B) and hemoglobin (C) with incubation in the culture supernatants of strains OSP1675 and OSP1689. Reaction mixtures (100 μl) contained culture supernatants from 40 μl culture and 30 μg of bovine serum albumin or hemoglobin in 50 mM Tris-HCl (pH 7.5). Incubation was carried out at 60°C for the indicated periods. Lanes M contain molecular mass markers.